Avi-tagged proteins use a 15-amino acid peptide (Gly-Leu-Asn-Asp-Ile-Phe-Glu-Ala-Gln-Lys-Ile-Glu-Trp-His-Glu) fused at either the N- or C-terminus of your target protein. This small tag enables highly specific enzymatic biotinylation by the BirA ligase under mild conditions, preserving native protein structure and function.https://www.betalifesci.com/pa....ges/avi-tagged-prote

Because biotin binds avidin/streptavidin with extreme affinity, Avi-tagged proteins can be immobilized reliably on surfaces like biosensors, microtiter plates, membranes, or chromatography matrices. This versatility makes them ideal for applications in protein purification, interaction assays, surface plasmon resonance, immunological studies, and high-throughput screening.