Large-scale production of recombinant proteins can be achieved in many expression systems. Bacterial expression systems offer the advantages of fast growth, well-characterized genetics, confirmed safety background, and high protein yield, making them one of the most important industrial expression hosts.
- Gene synthesis and codon optimization
- Vector construction
- Cloning cDNA into expression vectors
- Plasmid sequencing
- Plasmid preparation
- Protein expression testing
- Bacterial strain transformation
- Pilot expression and purification trial
- Protein expression evaluation (SDS-PAGE)
- Scalable expression and purification
- Soluble protein purification
- Protein refolding and purification (inclusion bodies)
- Large-scale expression and purification
- QC analysis (SDS-PAGE, UV, etc)
E. coli Expression Platform
E. coli serves as an attractive host system due to its well-characterized genetic background, high yield capacity, robust production cycle, strong anti-pollution abilities, and ease of scaling. Creative Biogene offers one-stop services for high-quality E. coli protein expression, with abundant experience in soluble protein expression and production and in protein refolding. We also offer extensive protein production and purification services tailored to your specific requirements, including endotoxin removal, tag removal, and protein labeling. Our E. coli cell culture in animal-free conditions, can be used to facilitate drug development. Pilot-scale production is available to enable the fast screening of therapeutic protein candidates.
B. subtilis Expression Platform
B. subtilis is a nonpathogenic bacterium that can efficiently secrete large quantities of a variety of enzymes into the culture medium. Such secretion facilitates further purification steps and simplifies product recovery. Many Processes concerning production technology and large-scale fermentation using this bacterium is very well acquainted. The major advantage of B. subtilis is that it does not produce LPS, and it has no significant bias in codon usage.
